Chromogen for enzyme beta-galactosidase is which of the following?

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Multiple Choice

Chromogen for enzyme beta-galactosidase is which of the following?

Explanation:
Chromogenic detection uses enzyme-substrate pairs to mark where the target is located. For beta-galactosidase, the best substrate is X-Gal, which the enzyme hydrolyzes to produce an insoluble turquoise (blue) pigment at the site of the target. The other chromogens are paired with different enzymes: DAB and AEC are substrates for horseradish peroxidase, giving brown or red signals, while Fast Red is typically used with alkaline phosphatase to yield red. Therefore, X-Gal yielding turquoise is the correct pairing for beta-galactosidase.

Chromogenic detection uses enzyme-substrate pairs to mark where the target is located. For beta-galactosidase, the best substrate is X-Gal, which the enzyme hydrolyzes to produce an insoluble turquoise (blue) pigment at the site of the target. The other chromogens are paired with different enzymes: DAB and AEC are substrates for horseradish peroxidase, giving brown or red signals, while Fast Red is typically used with alkaline phosphatase to yield red. Therefore, X-Gal yielding turquoise is the correct pairing for beta-galactosidase.

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