In HRP-based detection, which of the following is a common substrate used to generate a colorimetric signal?

Unlock all questions

This demo includes only 20 questions. Upgrade to access hundreds of questions, flashcards, exam simulations, and disable ads.

Full question bankExam simulationsFlashcards
From $25.99Unlock all

Master the QIHC Exam. Utilize flashcards and multiple-choice questions equipped with hints and explanations. Excel in your immunohistochemistry qualification!

Multiple Choice

In HRP-based detection, which of the following is a common substrate used to generate a colorimetric signal?

Explanation:
In HRP-based detection, the color you see comes from a chromogenic substrate that HRP oxidizes in the presence of hydrogen peroxide. Hydrogen peroxide is the oxidant that drives the reaction, not the color source. The most common color-producing substrates are 3,3'-diaminobenzidine (DAB) and tetramethylbenzidine (TMB), which yield brown and blue-to-yellow colors, respectively. Naphthol-AS-phosphate is used with alkaline phosphatase, not HRP. So the key idea is that a chromogenic substrate like DAB or TMB generates the color, with hydrogen peroxide simply enabling the reaction.

In HRP-based detection, the color you see comes from a chromogenic substrate that HRP oxidizes in the presence of hydrogen peroxide. Hydrogen peroxide is the oxidant that drives the reaction, not the color source. The most common color-producing substrates are 3,3'-diaminobenzidine (DAB) and tetramethylbenzidine (TMB), which yield brown and blue-to-yellow colors, respectively. Naphthol-AS-phosphate is used with alkaline phosphatase, not HRP. So the key idea is that a chromogenic substrate like DAB or TMB generates the color, with hydrogen peroxide simply enabling the reaction.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy