Which chromogen yields a blue color when used with alkaline phosphatase (AP)?

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Multiple Choice

Which chromogen yields a blue color when used with alkaline phosphatase (AP)?

Explanation:
Color development in immunohistochemistry depends on the enzyme label and the chromogenic substrate used. For alkaline phosphatase, certain substrates produce a blue precipitate when AP acts on them. Fast Blue BB is specifically designed for AP and yields a blue signal, which is why it’s the best match here. Other substrates with AP produce red colors (Liquid Fast Red, New Fuchsin) or brown with horseradish peroxidase (DAB). So the blue result aligns with using the Fast Blue BB chromogen for AP.

Color development in immunohistochemistry depends on the enzyme label and the chromogenic substrate used. For alkaline phosphatase, certain substrates produce a blue precipitate when AP acts on them. Fast Blue BB is specifically designed for AP and yields a blue signal, which is why it’s the best match here. Other substrates with AP produce red colors (Liquid Fast Red, New Fuchsin) or brown with horseradish peroxidase (DAB). So the blue result aligns with using the Fast Blue BB chromogen for AP.

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