Which of the following is a substrate associated with alkaline phosphatase in diagnostic assays?

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Multiple Choice

Which of the following is a substrate associated with alkaline phosphatase in diagnostic assays?

Explanation:
In diagnostic assays that use alkaline phosphatase as the enzyme label, the substrate must be one that ALP can hydrolyze to yield a detectable color. Naphthol-AS-phosphate fits this role because alkaline phosphatase removes the phosphate group, producing a naphthol derivative that then forms a colored product with a coupling reagent. The other substances are tied to horseradish peroxidase detection: hydrogen peroxide is a reactant in HRP-catalyzed reactions, while tetramethylbenzidine and DAB are chromogens that produce color when oxidized by HRP in the presence of hydrogen peroxide. So they’re not substrates for alkaline phosphatase.

In diagnostic assays that use alkaline phosphatase as the enzyme label, the substrate must be one that ALP can hydrolyze to yield a detectable color. Naphthol-AS-phosphate fits this role because alkaline phosphatase removes the phosphate group, producing a naphthol derivative that then forms a colored product with a coupling reagent. The other substances are tied to horseradish peroxidase detection: hydrogen peroxide is a reactant in HRP-catalyzed reactions, while tetramethylbenzidine and DAB are chromogens that produce color when oxidized by HRP in the presence of hydrogen peroxide. So they’re not substrates for alkaline phosphatase.

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