Which of the following is a substrate used by horseradish peroxidase in colorimetric assays?

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Multiple Choice

Which of the following is a substrate used by horseradish peroxidase in colorimetric assays?

Explanation:
The key idea is that horseradish peroxidase needs an oxidizing partner to drive color development. In colorimetric assays, hydrogen peroxide is the oxidant that the enzyme uses to oxidize a chromogenic substrate and generate a colored product. Substrates like diaminobenzidine or tetramethylbenzidine are the chromogens that get colored, but they rely on hydrogen peroxide to begin their oxidation. Naphthol-AS-phosphate is tied to alkaline phosphatase, not peroxidase. So the actual substrate consumed by the enzyme in this system is hydrogen peroxide.

The key idea is that horseradish peroxidase needs an oxidizing partner to drive color development. In colorimetric assays, hydrogen peroxide is the oxidant that the enzyme uses to oxidize a chromogenic substrate and generate a colored product. Substrates like diaminobenzidine or tetramethylbenzidine are the chromogens that get colored, but they rely on hydrogen peroxide to begin their oxidation. Naphthol-AS-phosphate is tied to alkaline phosphatase, not peroxidase. So the actual substrate consumed by the enzyme in this system is hydrogen peroxide.

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