Which statement describes an issue with dewaxing that can cause background staining?

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Multiple Choice

Which statement describes an issue with dewaxing that can cause background staining?

Explanation:
Incomplete dewaxing leaves paraffin in the tissue. That residual paraffin creates a hydrophobic barrier, preventing proper penetration of aqueous staining reagents and can trap chromogens, leading to nonspecific background staining. Fresh reagents or performing the antigen retrieval step are normal parts of the protocol and aren’t dewaxing failures; sections that are properly dewaxed should not show this background. So the issue described is the sections not being fully dewaxed, which best explains the background staining.

Incomplete dewaxing leaves paraffin in the tissue. That residual paraffin creates a hydrophobic barrier, preventing proper penetration of aqueous staining reagents and can trap chromogens, leading to nonspecific background staining. Fresh reagents or performing the antigen retrieval step are normal parts of the protocol and aren’t dewaxing failures; sections that are properly dewaxed should not show this background. So the issue described is the sections not being fully dewaxed, which best explains the background staining.

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