X-Gal is a chromogen used to detect which enzyme?

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Multiple Choice

X-Gal is a chromogen used to detect which enzyme?

Explanation:
X-Gal is a substrate that reveals beta-galactosidase activity. When this enzyme acts on X-Gal, it hydrolyzes the glycosidic bond and, after oxidation, forms a blue insoluble pigment that stains the cells expressing beta-galactosidase. This makes the positive cells stand out clearly. The other enzymes listed do not produce this blue color with X-Gal—horseradish peroxidase uses substrates like DAB to yield a brown/black precipitate, alkaline phosphatase uses BCIP/NBT to give blue/purple staining, and lysozyme is not a chromogenic substrate in this context.

X-Gal is a substrate that reveals beta-galactosidase activity. When this enzyme acts on X-Gal, it hydrolyzes the glycosidic bond and, after oxidation, forms a blue insoluble pigment that stains the cells expressing beta-galactosidase. This makes the positive cells stand out clearly. The other enzymes listed do not produce this blue color with X-Gal—horseradish peroxidase uses substrates like DAB to yield a brown/black precipitate, alkaline phosphatase uses BCIP/NBT to give blue/purple staining, and lysozyme is not a chromogenic substrate in this context.

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